International Sakharov Environmental Institute of Belarusian State University

• Organic Chemistry

• Chemistry and Biochemistry of Carbohydrates

• Methods of Structural Biology in the study of protein function

• Chemical and microbiological methods for the synthesis of nucleosides and nucleotides modified in the heterocyclic base and carbohydrate fragment have been developed.
• 5′-triphosphates of 3′-fluoro-2′,3′-dideoxynucleosides were synthesized and their high efficiency in inhibiting DNA synthesis under the action of DNA polymerases was shown.
• Methods have been developed for the synthesis of the bioregulator-trimmer 2′, 5′ – oligoadenylate, which is one of the intermediates in the mechanism of antiviral action of interferon, as well as its 2′, 3′ – riboanhydro analog, which exhibits immunomodulatory properties and prevents tissue rejection in transplantation operations.
• Methods for the synthesis of selectively blocked nucleotides containing photosensitive protective groups based on thioxanthone derivatives are developed; the possibility of using the obtained compounds for constructing high-density DNA biochips is shown.
• Chemical and microbiological methods for the synthesis of nucleosides and nucleotides modified in the heterocyclic base and carbohydrate fragment have been developed.
• 5′-triphosphates of 3′-fluoro-2′,3′-dideoxynucleosides were synthesized and their high efficiency in inhibiting DNA synthesis under the action of DNA polymerases was shown.
• Methods have been developed for the synthesis of the bioregulator-trimmer 2′, 5′ – oligoadenylate, which is one of the intermediates in the mechanism of antiviral action of interferon, as well as its 2′, 3′ – riboanhydro analog, which exhibits immunomodulatory properties and prevents tissue rejection in transplantation operations.
• Methods for the synthesis of selectively blocked nucleotides containing photosensitive protective groups based on thioxanthone derivatives are developed; the possibility of using the obtained compounds for constructing high-density DNA biochips is shown.

Implementations

The patented original antiviral drug “Nucleavir” as well as the antitumor drug “Fludarabine phosphate” produced at the Minsk RUE “Belmedpreparaty”.

He has more than 120 scientific papers, including more than 40 publications in English, 3 textbooks, 44 USSR Copyright certificates for inventions, 7 patents (USA, GDR, FRG, Republic of Belarus and Eurasian patent):

1. 1. Z.G. Chedgeavadze, A.V. Skamrov, R.Sh. Beabealashivilli, E.I. Kvasyuk, G.V. Zaitseva, I.A. Mikhailopulo, G. Kowollik, P. Langen. 3′-Fluoro-2′,3′-dideoxy-β-D-erythro-pentofuranosylnucleoside 5′-triphosphates – terminators of DNA synthesis, catalyzed by DNA Polymerases // FEBS Letteers. 1985. V. 183, №. 2, 275-278.
   2. E.I. Kvasyuk, T.I. Kulak, N.B. Khripach, I.A. Mikhailopulo, E. Uhlmann, R. Charubala, W. Pfleiderer. Nucleotides XXIV. Preparative synthesis of trimeric (2′-5′) oigoadenylic acid // Synthesis. 1987, №6, 535­541.
   3. 3. A.I. Zinchenko, V.N. Barai, S.B. Bokut, E.I. Kvasyuk, I.A. Mikhailopulo. Synthesis of 9-(β-D-arabinofuranosyl)guanine using whole cells of Escherichia coli           // Appl. Microbiol. Biotechnol. 1990. V. 32, №3, 658-661.
   4. I.A. Mikhailopulo, N.E. Poopeiko, T.I. Prikota, G.G. Sivets, E.I. Kvasyuk, J. Balzarini, E. DeClercq. Synthesis and antiviral and cytostatic properties of 3′-deoxy-3′-fluoro-and 2′-azido-3’fluoro-2′,3′-dideoxy-D-ribofuranosids of natural heterocyclic bases // J. Med. Chem. 1991. V. 34, №7, 2195-2202.
   5. E.I. Kvasyuk, T.I. Kulak, I.A. Mikhailopulo, R. Charubala, W. Pfleiderer. Nucleotides. Part XLV. Synthesis of new (2′-5′) Adenylate Trimers, containing 5’Amino-5′-deoxyadenosine Residues at the 5′-End of the Oligoadedenylate chain, and Its Analogues, Carrying a 9[(2-Hydroxy-ethoxy)methyl]adenine Residue at the 2′-Terminus // Helv. Chim Acta. 1995. V. 78, №5, 1777-1784.
   6. E.I. Kvasyuk, T.I. Kulak, O.V. Tkachenko, S.L. Sentyureva, I.A. Mikhailopulo, R.J. Suhadolnik, E.E. Henderson, S.E. Horvath, Ming-Xu Guan, W. Pfleiderer. Synthesis and biological activity of new sugar modified 2′,5′-oligoadenylate trimers // Nucleosides and Nucleotides. 1997. V. 16, № 5/6, 773-776.
   7. H. Giegrich, S. Eilese-Bühler, Chr. Hermann, E.I. Kvasyuk, R. Charubala, W. Pfleiderer. New Photolabile Protecting groups in nucleoside and nucleotide chemistry-synthesis, cleavage mechanisms and applications // Nucleosides and Nucleotides. 1998. V.17, № 9/11, 1987-1996
   8. C. Hermann, E. Kvassiouk, W. Pfleiderer. Nucleotides Part LXXIX. New building blocks for photolithographic synthesis of oligonucleotides // Helv. Chim. Acta. 2011. V. 94, № 3, 362-370.
   9. A.S. Drazdou, A.N. Sobchuk, V.E. Syakhovich, O.S. Bokut, E.I. Kvasyuk, B.A. Bushuk, S.B. Bokut. Fluorescence decay time distribution of 1-anilinonaphthalene-8-sulfonate in human oxyhemoglobin A_1b solution // J. Appl. Spectrosc.  2012.  V. 79, № 3, 437-445.
   10. А.И. Береснев, Е.И. Квасюк, С.В. Квач, А.И. Зинченко. Атака на теломеры, или Подход к терапии рака с помощью 6-тио-2’-дезоксигуанозина // Наука и инновации. 2016. № 9 (163). 58-61.
2. 11. А.И. Береснев, А.Н. Рымко, Л.А. Ерошевская, С.В. Квач, Е.И. Квасюк, А.И. Зинченко. Синтез флударабин-5′-монофосфата с использованием бактериальных рекомбинантных ферментов // Весцi Нацыянальнай акадэмii навук Беларусi. Серыя бiялагiчных навук. 2017. № 1. С. 7-15.
   12. А.И. Береснев, Е.И. Квасюк, А.И. Зинченко. Синтез 2’-дезоксиизогуанозина с использованием бактериальнвх нуклеозидфосфорилаз // Микробные биотехнологии: фундаментальные и прикладные аспекты: сб. науч. тр. / Институт микробиологии НАН Беларуси; под ред.: Э.И. Коломиец и А.Г. Лобанка. – Минск: Беларуская навука. 2018. Т. 10. С. 299-305.
   13. М.В. Лисовская, Е.И. Квасюк. Синтез эфиров эмоксипина // Журн. Белорус. гос. ун-та. Экология. 2018. № 4. С. 119-127.

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